ALP

PURPOSE :
The ALKALINE PHOSPHATASE (ALP) LIQUID REAGENT is, a kinetic method, used for the quantitative determination of alkaline phosphatase (ALP) in serum.
 
PRINCIPLE :
.Alk ...Phos                 
p-NPP + H2O -----------------------------> p-Nitrophenol + H3PO4
p-Npp is colorless but p-Nitrophenol has b absorbance at 405 nm. The rate of increased absorbance at 405nm is proportional to the enzyme activity.


 
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