 TOTAL CHO |
|
| PURPOSE : |
| The CHOLESTEROL LIQUID REAGENT is an end point method , used for the quantitative determination of total cholesterol in serum. |
|
| PRINCIPLE: |
| C. Esterase |
| Cholesterol Esters -------------------------> Cholesterol + Fatty acids |
| C. oxidase |
| Cholesterol + O2 --------------------------> Cholesten-3-one + H2O2 |
| H. peroxidase |
| 2 H2O2 + 4-Aminoantipyrine + p-HBS ----------------------> Quinoneimine + 2 H2O |
| |
| Cholesterol esters are hydrolyzed to produce cholesterol. Hydrogen peroxide is then produced from the oxidation of cholesterol by cholesterol oxidase. In a coupled reaction catalyzed by peroxidase, quinoneimine dye colored red is formed from 4-aminoantipyrine, p-HBS and hydrogen peroxide. The absorption at 520nm of the solution of this dye is proportional to the concentration of cholesterol in the sample. |
|
|
 |
