CSF & URINE PROTEIN

PURPOSE:
The CSF & URINE PROTEIN is, an end point method, used for the quantitative determination of total protein in cerebrospinal fluid (CSF) or urine.
 
PRINCIPLE :
Protein +Coomossie brillant blue dye ---------> Protein-dye complex
 
The CSF and urine protein binds selectively to the dye coomossie brillant blue. The increase in absorbance of the resulting protein-dye complex, read at 600 nm, is proportional to the protein concentration.

 
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