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 TG |
| PURPOSE : |
| The TRIGLYCERIDES GPO LIQUID REAGENT is, an end point method, used for the quantitative determination of triglycerides in serum. |
| PRINCIPLE : |
| Lipase Triglycerides -----------------------------------------------> Glycerol + Fatty acids |
| Glycerol Kinase Glycerol + ATP --------------------------------------> Glycerol-1-phosphate + ADP |
| GPO Glycerol-1-phosphate + O2 ----------------------------------> DAP + H2 O2 |
| .Peroxidase H2 O2 + 4-AA + DHBS ------------------------------------> Quinoneimine(Red) + 2H2 O |
| The present procedure involves hydrolysis of triglycerides by lipase. The glycerol concentration is then determined by enzymatic assay coupled with Trinder reaction that terminates in the formation of a quinoneimine dye. The amount of the dye formed, determined by its absorption at 520nm, is directly proportional to the concentration of triglycerides in the samples. |
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