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 TOTAL PROTEIN |
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| PURPOSE : |  |
| The TOTAL PROTEIN (Biuret reagent) is, an end point method, used for the quantitative determination of total protein concentration in serum. | |
| PURPOSE : | |
| Alkaline solution Protein + Cu ----------------> Cu-protein complex (Blue) |
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| Protein in serum forms a blue colored complex when reacted with curpic ions in an alkaline solution. The intensity of the violet color is proportional to the amount of protein present when compared to a solution with known protein concentration. | |
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