 URIC ACID |
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| PURPOSE : |
| The URIC ACID LIQUID REAGENT is an end point method, used for the quantitative determination of uric acid in serum. |
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| PRINCIPLE : |
| ......Uricase |
| Uric acid+ O2 + 2H2O ----------------------> Allantion + CO2 + H2O2 |
| .Peroxidase |
| 2 H2O2 + 4-Aminopyrine + DHBS -------------------> Chromogen + 4H2O |
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 Uric acid is converted by uricase into allantoin and hydrogen peroxides. The hydrogen peroxide initiates the coupling of 4-aminoantipyrine to 3,5-dichloro-2- hydroxybenzene sulfonic acid (DHBS) to form the chromogen which is measured at 520 nm and which is proportional to the amount of hydrogen peroxide generated from Uric Acid. |
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