RAPID GRAM STAIN

PURPOSE :
The SPUTUM PRETREATMENT SOLUTION is used for the concen-tration and inactivation of Mycobacterium tuberculosis (T.B.)/Acid Fast Bacilli in human sputum.
 
PRINCIPLE :
In the past, sputum specimen was directly used to make a direct smear for the stain of T.B. in the bacterial laboratory. The accuracy and discover was lower. Statistically, the discovery rate of positive specimens from direct smear was about 30-40%. Furthermore, during the procedure of heating step, T.B. in the positive sputum specimen will vapor into air. The vapor T.B. may infect the operator or people in the bacterial laboratory.
 
SPUTUM PRETREATMENT SOLUTION :
can offer the following advantages
a. Clear background: viscosity, epithelium cell and other bacterial became soluble.
b. Inactivation: the infectious of T.B. was inactivated.
c. High sensitivity: after concentration, the discovery rate increased.
d. High specificity: no false negative was found.

 
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