ACID FAST STAIN(COLD STAIN)

PURPOSE :
The ACID FAST STAIN (COLD STAIN) is a stain solution , used for the staining of Mycobacterium tuberculosis/Acid Fast Bacilli.
 
PRINCIPLE :

Phenol appears to combine fuchsin basic dye within Acid Fast Bacilli . When stain is applied, all cells including hard to stain Acid Fast, are colored red . All tissue components except the Acid Fast Bacilli are then decolorized . After counterstaining, all decolorized tissue components are colored blue.

 
 

 
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